Culture conditions for callus induction and anthocyanin accumulation of Celosia cristata 'phumosa' were studied in this paper. The induction rate, callus growth and anthocyanin accumulation of different explants isolated from the seedings in different age were compared for the selecting of rapid-growing and steadily-anthocyanin-accumulating lines. Two callus lines JSA10 and JSA25 were established and the former was used to investigate the factors regulating callus growth and pigmentation. The ma...