玉米小斑病(Southern blight of corn)是当前国内外玉米生产区普遍流行的一种真菌性病害,在致病过程中能产生对其寄主植物具有致病活性的代谢产物,即致病毒素。本研究首先从玉米小斑病菌C小种致病力的恢复、HMC毒素的产生与培养条件的关系、玉米小斑病菌C小种致病过程与钙信号系统的关系、HMC毒素对同核异质玉米叶肉细胞原生质体的影响四个方面对玉米小斑病菌C小种致病特性进行了研究。结果表明:玉米小斑病菌C小种可采用PDA培养基与寄主叶片连续共培养的方法提高产孢量和致病力;黑暗、静止培养有利于毒素的产生;毒素的提取过程中,洗脱液的改进提高了毒素的纯度,相应地提高了毒素的致病力;钙信号途径抑制剂EGTA、Verapamil、KN-93对玉米小斑病菌分生孢子萌发和附着胞的形成过程具有抑制作用,说明钙信号途径参与了小斑病菌分生孢子萌发和附着胞的形成过程,且这种参与表现在孢子萌发和附着胞形成过程的早期;HMC毒素对C细胞质玉米叶肉细胞原生质体具有破坏作用,可使原生质体破裂、瓦解,而对N细胞质玉米叶肉细胞原生质体的破坏作用不明显,说明玉米小斑病菌C小种对C细胞质玉米的原生质体具专化破坏作用...
【英文摘要】
Southern blight of corn was a king of universal fungal plant disease at the present.
During infecting corn seedings, the pathogen of Bipolaris maydis race C could produce an active metabolic production, namely pathogenic toxin (HMC-Toxin). We studied the pathogenic characteristic of Bipolaris maydis race C on pathogenicity restoration, on the relation between Ca2+ signal pathway and conidium germination and appressorium formation, on the relation between the production and cultural condition of H...