In order to study the optimum introducing period, survival rate and inversion rate, the
technique of pollen tube path way was used to introduce insect-resistant and disease-resistant gene into soybeans. PCR was also done to test the Dland D2 generations of transgenic plants. All these have proved that the technique of pollen tube path is feasible to study soybean.
The results showed that:
1. The way of drop of liquid was used to introduce chitinase and Bt gene into 27 soybea...