Anemone flaccida Fr. Schmidt belongs to Anemone. Generally, the plant is called as Diwu simply, and also the dry rhizoma of A.flaccida is called as'Diwu'in the native medicine, which was used to confront inflammatory and pain. To optimize the rapid-proliferation in vitro of A.flaccida, this thesis focused on the effect of factors which affected the tissue culture growth stages, such as adventitious bud proliferation, CLS induction, rooting and acclimatization and transplants.The quantitative and qua...