Cloning of SRY Gene from Moschus Berezovskii and Moschus Chrysogaster and the Prediction and Analysis of SRY Protein's Structure and Its Application in Phylogenetic Analysis
CDS region of SRY gene were cloned from M. berezovskii and M. chrysogaster genomic DNA by PCR method with primers according to the known SRY sequences from Artiodactyla animals.The nucleotides sequences of SRY CDS were translated into amino acid sequences and were analyzed using softwares. It was found that there have a high nonsynonymous sbustitutions in HMG-box region between the M. berezovskii and M. chrysogaster, which suggested that in some degree the HMG-box is affected by the positive directional sel...
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