In this experiment, the effect of 6-Benzyladenine (6-BA) on callus inducement and regeneration from mature embryos of 253 and Bo-B, and the physiological and biochemical characteristics during the callus inducement and differentiation were studied .The results were showed as follows:
(1)Induced medium containing of 6-BA and 2,4-D can remarkably improve the induction rate and the quality and the regeneration rate of callus from mature embryos .Established a high frequency regeneration system.
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