17 pollinations between seedless grapes andChinese wild grapes (K amurensis (Heilongjiang seedling,Zuoshan741-326) V.thunbergii(raKhanr2),V.yeshanensis,V. qinlingensis (Pingli-5) and Beichun (a specific hybrid of V.amurensisJ), 6 pollinations between seedless grapes, 2 crosses of self-pollinated seedless grapes were carried out in May of 2000 and May of 2001.Fertilized ovules of 25 crosses above were cultured in vitro to study effect of harvest time, basic medium, embryo culture styles, ovule ex...