合欢皮(Cortex Albizziae)为豆科植物合欢Albizzia julibrissin Durazz.的干燥茎皮。其味甘,性平。有安神、活血、消痈的功能。在传统医学中用于失眠、肺脓疡、痈肿、折伤疼痛等疾病的治疗。现代药理学研究表明,皂苷为合欢皮的主要有效成分,具有广泛的生理活性和药理作用。本课题从中药合欢皮中分离提取总皂苷,并以大孔吸附树脂进行进一步分离,检测了合欢皮总皂苷AJS及其5个流份的溶血性,研究了总皂苷AJS及其流份对卵清蛋白(OVA)和禽流感鸡痘病毒载体活疫苗(rFPV-HA)的佐剂活性,评价了它们的佐剂作用和特点,确定AJS佐剂活性的主要有效部位,为合欢皮皂苷作为免疫佐剂开发研究提供了科学依据。
1.合欢皮总皂苷及其5个流份的溶血性
采用比色法测定AJS及其5个流份(FW、FE30、FE50、FE75和FE95)对0.5%兔红细胞的溶血性。根据引起红细胞50%溶血浓度(HD50值),合欢皮总皂苷及其5个流份的HD50均显著大于Quil A (P<0.001)。5个流份中以FE75的溶血性最强,其HD50为7.87±0.30μg·mL-1;其次FE9...
【英文摘要】
The stem bark of Albizia julibrissin Durazz, Hrhuanpi, is a well-known traditional Chinese medicine used to to relieve melancholia and uneasiness of body and mind, to invigorate the circulation of blood and subside a swelling. Extensive phytochemical and pharmacological studies on this plant proved the saponins to be the main bioactive principles. With the aim of selecting non-haemolytic saponins which could be possibly used as adjuvant in future large-scaled studies of vaccination, in this thesis, ...