烟草黑胫病(tobacco black shank)在我国分布范围很广,除黑龙江省尚未发现之外,其余各植烟省区,包括台湾在内均有发生。烟草黑胫病菌(Phytophthoranicotianae)可以危害烤烟、晾烟、晒烟、白肋烟、香料烟等所有的栽培烟草,破坏力极强。
1.菌株来源
本研究从重庆主产烟区采集典型烟草黑胫病样品,分离、纯化得到12株烟草黑胫病菌菌株,加上由西南大学植物生态病理研究所提供的50株烟草黑胫病菌菌株,共62株。菌株来源覆盖重庆市酉阳、黔江、彭水、武隆、涪陵、万州、巫溪、巫山8大主产烟区。
2.菌株鉴定
分离到的烟草黑胫病菌菌株主要培养性状为:菌落无色,菌丝无隔,孢子囊多近球形,乳突明显,具脱落性,且孢子囊柄短,不对称孢子囊不常见;对烟草具有致病性。并通过聚合酶链式反应(PCR)对各地代表菌株的核糖体DNA内转录间隔区(rDNA ITS)的特异性扩增,得到了长度为892bp的特异性片段,经BLAST比对后,与烟草黑胫病菌的亲缘关系达到99%,明确供试菌株为烟草疫霉。
3.交配型测定
采用培养皿配对培养的方法...
【英文摘要】
The distribution of tobacco black shank was very extensive in China,and it was found in almost all the provinces where were planting tobacco including Taiwan,except Heilongjiang.Phytophthora nicotianae,the casual agent of tobacco black shank, infected all the tobacco cultivars including flue-cured tobacco,air-cured tobacco, sun-cured tobacco,burley tobacco,oriental tobacco and brought powerful destroy.
1.The source of isolates
The samples with typical symptom of tobacco black shank were ...