Stevia glycosides, a kind of mixture which contains eight diterpene glycosides present in stevia rebaudiana Bertoni. As a new natural sweetener, it is low caloric, high sweet and notoxicty, so it is a very perfect substitute for sucrose. Reliable separation and determination of the stevia glycoside is crucial, because the taste of each Stevia glycoside is different. This paper studied the method to detect and separate the stevia glycosides, a new capillary zone electrophoresis (CZE) separation method...