The positive control was obtained from the samples reserved in our laboratory by PCR method.The negative control is the DNA extract from E.coli ATCC25922.A multi-PCR detection method was established.In order to fred out the best reaction parameter we optimized Mg~(2+)、dNTPs、Taq enzyme and primers concentration,the best annealing temperature and the best cycle number of times.The final reaction system was shown as follows:10×PCR Buffer 5μL,MgCl_2 (25 mmol/L) 3.0μL,dNTPs(2.5 mmol/L) 4μL,DNA template 5μ...
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