Construction of Four Color Fluorescence-Labeled Multiplex Typing System for Six MiniSTR Loci D9S1122/D10S1435/D12ATA63 and D20S1082/D18S853/D17S1301 and Evaluation of Its Forensic Application
目的:短串联重复序列(short tandem repeats, STR)又称微卫星DNA,是由1-6个碱基串联重复而成的DNA序列。STR绝大部分分布于人类基因组非编码区,少数分布在编码区,平均每6-10kb就有一个STR基因座。由于STR基因座在人类基因组中分布广泛,具有高度的遗传多态性以及简单快捷的检测方法而被广泛应用于遗传制图、遗传连锁性分析、肿瘤研究、人类学、法医学、疾病基因定位、物种多态性研究等诸多领域。在法医DNA检验中,STR技术已经广泛用于个人识别和亲权鉴定方面。但对于法医学中一些微量降解的检材,STR技术并不能成功地得出结论,经常会出现“优势扩增”或者“无效扩增”,而miniSTR分析技术是将引物设计得尽可能靠近核心序列来缩短扩增片段长度,因此将其用于高度降解DNA样本检测可提高个人识别和亲子鉴定中的检测成功率。MiniSTR基因座已被欧洲DNA分型工作组(EDNAP)定义为继STR之后的新一代遗传标记,2006年美国AB公司已经研制开发了miniSTR扩增试剂盒;美国、日本、西班牙等国家报道了miniSTR D1S1677、D4S2364、D10S1248等基因座的群体遗传学...
【英文摘要】
Objective: Short tandem repeats (STR), also named microsatellite DNA, is a kind of genetic marker consisted of 1~6bp tandem repeats. Most of the STR loci locate in the non-coding regions of human genome, and the few is in the coding region. STR has been studied and applied in many fields such as phymatology, anthropology, hereditism, and forensic science, etc., because of its extensive distribution in the genome, high polymorphism and simple analytic methods. At present, STR has been used as a helpfu...
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