L gene of ClassⅠ9a5b virulent strain was amplified by RT-PCR and cloned to pGEX-6P-1 expression vector.After sequencing,the recombinant plasmid was transformed into BL21 which were then induced by IPTG to produce a recombinant protein of 39 ku.BALB/c mice were immunized with the purified expression product of L protein.The ELISA,indirect immunofluorescence assay IFA and Western-blot analysis were used to characterize and identify antibodies.Results showed that a panel of 4 hybridoma cell lines were successf...
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