Objective To prepare colistin sulphate liposomes and study on preparation processes.Methods Colistin sulphate liposomes were encapsulated into the liposomes using active loading that pH gradient method,pH gradient method coupled with reverse evaporation technique,free colistin sulphate and liposomes were separated by ultrafiltration.HPLC was applied to determine the entrapment efficiency(EE).The influence of various factors on EE were investigated.Results The optimum formula and preparation conditions were ...
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