To construct eukaryotic expression vector of general type in reverse genetics systems of non-segmented negative strand RNA viruses,the multiple cloning site of the plasmid pVAX1 was replaced with HamRz cDNA sequence,a 9 sites linker and HdvRz cDNA sequence through the sequential addition of three adapters,the insertion of which could generate the correct 3' and 5' terminal sequences of the primary viral genomic RNA transcript and facilitate the assembly of the complete viral cDNA sequence.The sequences of t...
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