[Objective] The research aimed to provide the theoretical basis and the technical reserve for the genetic engineering breeding of sugarcane.[Method] Total RNA was extracted from tender leaves of sugarcane.Two pairs of primers were designed according to cDNA sequence of ACC oxidase(ACO) on GenBank website to amplify ACO cDNA sequence in sugarcane.The sense and antisense plant expression vectors of ACO cDNA in sugarcane were constructed.And the recombined plant expression vectors containing ACO gene was used ...
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