[Objective] The research aimed to study the polymorphism and cluster analysis of Cyathula officinalis Kuan.,Cyathula capitata(Wall)Moq and their hybrid accession by RAPD and AFLP marks.[Method] General DNA was isolated from the fresh leaves of target plants by phenol-chloroform method.RAPD and AFLP markers were applied and determined by PCR and gel electrophoresis.The determined molecular markers were analyzed though UPGMA method of software NTSYSpc-2.10s.[Result] RAPD markers were more efficiency than AFLP...