Objective: Determine the total flavonoids and activity of PAL after adding SAlicylic acid.Methods: The total flavonoids were extraceted by Soxhlet extraction and determined by spectrophotometry.The activity of PAL also determined by spectrophotometry.Results: SA in the range of 50μmol/L-150μmol/L can enhance the total flavonoids contents and activity of PAL in culture Gynostemma.Conclusion: Adding slicylic acid can apply in enhancing the total flavonoids contents in culture Gynostemma.