According to the biodegradation of white rot fungi and the application of Laccase to environment and industry, the condition of producing Laccase by Coriolus Versicolor was studied. The optimum culture components were: 1.8?g/L Maltose, 0.84?g/L Ammonium Tartrate, 100?mL/L Massive elements solution, 25?mL/L Trace elements solution, 100?μg/LVB 1, 0.05?% Tween 80. The better induction material was Dimethylaniline and the optimum pH of production Laccase was 3.6.