A pheA gene resistant to phenylalanine feedback was isolated from a chemically mutated E.coli strian and cloned into a shuttle plasmid pLCX36 from E.coli to coryneform bacteria, resulting a chimeric expression plasmid pLCX37.pLCX37 was transferred by conjugation into fluorophenylalanine resistant strains of B.flavum 3621 and so on. One of the transconjugants, 3621.09 had shown the highest yield of phenylalanine, up to 26.26mg/ml, about two times increased, if compared with that of the parent st...
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