Purpose:This study was to separation and purification of Bletilla Striata polysaccharide with modified method. Material and method:Crude polysaccharide was prepared by ethanol precipitation followed the60℃water extraction, deproteinized with Savage method, petroleum ether defatted and activated carbon bleaching. It was further isolated by ion-exchange chromatography on DE-52column and gel filtration on Sephadex G-100column and obtained purified Bletilla Striata polysaccharide. Result:The Bletilla Striata po...