以爪哇伪枝藻(Scytonema javanicum (Kütz.) Born et Flah)、具鞘微鞘藻(Microcoleus vaginatus Gom.)和纤细席藻(Phormidium tenue (Menegh.) Gom.)为材料,研究了前两种荒漠藻在紫外线(UV)辐射下的形态和超微结构、生理生化特性的变化以及它们消除因UV辐射所导致的氧化胁迫的机理;还对三种荒漠藻规模化培养条件进行了优化,并对它们藻结皮的培植与发育、藻结皮发育阶段理化性质的改变等进行了细致地研究。
经UV辐射后,S. javanicum细胞的直径和体积变小。在增强的UV-B辐射下,培养物变黄且生物量下降,藻细胞沉于液体培养基底部;而在UV-A辐射下,培养物浓度增加,藻细胞浮于液面。通过电镜发现,经UV辐射后,藻细胞中脂肪颗粒和多糖颗粒等与对照相比都有所增加;经UV-A处理后,藻细胞内片层结构更明显,几乎充满整个细胞;在UV-B处理后的藻细胞横切面周边区有一圈透亮小点。
对S. javanicum和M. vaginatus的光合作用研究发现,在低剂量(低于0....
【英文摘要】
Scytonema javanicum (Kütz.) Born et Flah, Microcoleus vaginatus Gom. and Phormidium tenue (Menegh.) Gom., isolated from desert microbial crusts by Dr. C.X. HU, were used as experimental materials. Effects of UV radiation on the morphology and ultrastructure of S. javanicum, and on the physiological and biochemical characteristics of S. javanicum and M. vaginatus were studied. Those that the mechanism of oxidative stress by UV-B radiation, the optimized cultivation conditions for mass culture, th...