A Study on Tissue Culture and Rapid Propagation of Parakmeria Lotungensis (Chun et C.Tsoong) Law、Sinomanglietia Glauca Z.X.Yu et Q.Y.Zheng and Photinia× Fraseri 'Red Robin'
本研究以国家二级保护、濒危树种乐东拟单性木兰Parakmeria lotungensis(Chun et C.Tsoong)Law,江西特有的国家一级保护树种、木兰科单种属华木莲Sinomanglietia glauca Z.X.Yu et Q.Y.Zheng及现行珍稀彩叶观赏树种红叶石楠‘红罗宾’Photinia×fraseri 'Red Robin'为试验材料,利用细胞全能性及其发育调控理论,建立三树种的组织培养再生体系。通过细胞学观察及与生根相关的酶活性分析,探讨红叶石楠的组培再生机理。探索生物防腐剂对组培过程中污染的抑制作用。结合设施园艺栽培技术,有针对的研究林木组培规模化育苗技术体系。本研究取得的主要结果:
1、以成年树嫩枝为外植体建立了乐东拟单性木兰的组培再生系统。10月上旬采取嫩枝作外植体,经4℃冰箱低温处理5~7d,有利于降低褐化和污染率,提高诱导率。试验筛选以MS为基本培养基,较好增殖培养基为:MS+6-BA0.2mg/L(单位下同)+IBA0.05+30g/L糖+7g/L卡拉胶;pH值范围为5.5~6.5;光照强度控制在10001x左右;继代周期以4周为宜,继代9...
【英文摘要】
The secondary class national preserved and endangered plant Parakmeria lotungensis (Chun et C.Tsoong)Law, The first class national preserval plant Sinomanglietia glauca Z.X.Yu et Q.Y.Zheng endemic in Jiangxi province, and the up-to-date and rare color-leaf plant Photinia fraseri 'Red Robin' were respectively studied to establish tissue culture and rapid propagation system based on plant cell totipotency and its regulation and control theory. Meanwhile, the regeneration mechanism of tissue culture of P. fras...