【作者】 楚金普； 郝玉庆； 李继遥； 周学东；

【Author】 CHU Jin-pu, HAO Yu-qing, LI Ji-yao ,ZHOU Xue-dong.(Key Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University;Lab of Caries Research, West China College of Stomatology, Sichuan University.Chengdu 610041,Sichuan Province,China)

【机构】 口腔生物医学工程教育部重点实验室； 四川大学华西口腔医学院龋病研究室；

【摘要】 目的:观察pH循环后五倍子化学成分对早期龋表面形态变化的影响,了解五倍子化学成分对人工早期釉质龋的再矿化作用。方法:应用离体牛切牙制备人工釉质龋标本60例,随机分为6组:(a)NaF组(阳性对照)、(b)GCE组、(c)GCE-B组、(d) GCE-B1组、(e)GCE-B2组和(f)去离子水组(阴性对照),进行体外pH循环实验后,各组分别取四个样本放入2．5％戊二醛液内固定24小时、4℃,临界点干燥,镀金,扫描电镜下观察。结果:再矿化后,NaF组的釉质表面变的比矿化前更加规则、均匀的外观,有许多圆形或椭圆形的晶体颗粒沉积,大颗粒常由数个小颗粒组成,密集、紧凑,略显隆起。GCE组、GCE-B组、GCE-B1组的釉质表面有一层似膜状的结构形成,有一定厚度,进一步观察这层膜结构是由一些呈小球状或不规则的颗粒组成,但外观显得相对不如氟化物组那样规则、均匀。而GCE-B2组釉质表面没有明显的沉积物形成,其外观与去离子水组相似,可见釉柱中心发生脱矿所形成的凹坑。结论:五倍子化学成分:GCE、GCE-B和GCE-B1具有潜在的促进早期龋再矿化的功效。更多还原

【Abstract】 OBJECTIVE: To evaluate the effect of chemical compounds of Galla chinensis on the remineralization of initial enamel carious lesions by scaning electron microscope in vitro. METHODS: Sixty bovine enamel blocks with early lesions were prepared and randomly divided into six-treatment groups.the lesions were subjected to the pH-cycling regime for 12 days. Each daily cycle included 4×1 minute applications with one of six treatments; 1,000ppm F aq. (as NaF, positive control); deionized water (negative control); or 4,000 ppm aqueous solutions of four Galla Chinensis extracts (GCEs); GCE, GCE-B, GCE-B1, or GCE-B2. After pH-cycling,the enamel specimens was observed by scaning electron microscope. RESULTS: The results showed that there were sediments formed on the surface of carious lesion for the positive control, GCE, GCE-B and GCE-B1 groups except the GCE-B2 group and DDW group. CONCLUSION: Galla chinensis might still be a promising source of non-fluoride anti-caries agents.更多还原