【作者】 郑国香； 任南琪； 钟溢键； 李小玲； 吴川福； 周湘良；

【Author】 ZHENG Guo-xiang, REN Nan-qi, ZHONG Yi-jian , LI Xiao-ling, WU Chuan-fu , ZHOU Xiang-liang (School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090,China)

【机构】 哈尔滨工业大学市政环境工程学院；

【摘要】 利用间歇实验对不同碳源、氮源及碳氮质量比值对产氢细菌RF-9的发酵产氢行为影响进行了探讨。结果表明,葡萄糖和玉米浆+酵母膏复合物是产氢菌RF-9发酵产氢较理想的碳源和氮源。碳／氮(mC／mN)质量比值对菌株RF-9的生长发酵产氢行为产生很大的影响,随着mC／mN比值的逐渐加大,菌株RF-9的生长及发酵产氢性能也逐渐提高,当mC／mN为5．5时,葡萄糖利用率最大93．3％,产氢菌株RF-9的释氢量和生长量也达到最大,分别为135．9 mmol／L和0．723 g／L,发酵液相末端产物总量相应地达到最高5 850．8 mg／L,其中乙醇和乙酸是主要的可溶性代谢产物,pH降为最低3．36。当mC／mN>5．5,随着mC／mN比值的继续增加,菌株RF-9的生长发育及代谢性能表现为下降趋势。更多还原

【Abstract】 The influence of different carbon, nitrogen sources and carbon/nitrogen mass ratio on H2-producing of strain RF-9 were studied in this work in batch test. The results showed that the composite of glucose,corn steep olquor and yeast extract paste were the optimal carbon and nitrogen sources of H2-producing strain RF-9. The carbon-nitrogen mass ratio (mC/mN) has a significant influence on fermentative hydrogen production capacity of strain RF-9, cell growth and fermentative H2-producing ability would be improved with increasing mC/mN value. When the value of mC/mN was 5.5, utilization ratio of glucose,release of H2 and cell concentration reached maximal value which were 93.3% , 135.9 mmol/L and 0.723 g/L, respectively. Moreover, the total concentration of fermentative liquid end products obtained correspondingly maximal value of 5 850.8 mg/L, among which ethanol and acetic acid were main soluble metabolites,the pH of culture medium dropped to the minimum value of 3. 36. When mC/m N exceeded 5.5, cell growth and metabolic characteristic of strain RF-9 were restrained and showed dropping tendency.更多还原