【摘要】 目的观察调肝解毒方对慢性癫痫大鼠学习记忆与海马组织环磷酸腺苷反应元件结合蛋白(CREB)表达的影响。方法60只大鼠腹腔注射印防己毒素制作慢性癫痫模型后,随机分为模型组,调肝解毒方低剂量组、调肝解毒方高剂量组,丙戊酸钠组,每组15只,与正常组(15只)同期观察或治疗60天,以Morris水迷宫观察大鼠空间学习和记忆能力,应用RT-PCR方法检测大鼠海马组织CREBmRNA表达变化,免疫组织化学方法检测海马组织磷酸化的CREB水平变化。结果调肝解毒方低、高剂量组大鼠空间记忆能力高于模型组(P<0.01),海马组织CREBmRNA相对吸光度值增高,pCREB免疫阳性细胞的表达有不同程度增强,与模型组比较差异有显著性意义(P<0.05,P<0.01)。结论调肝解毒方可在一定程度上改善反复癫痫大鼠的学习记忆能力,可能与调节海马CREB表达有关。更多还原

【Abstract】 Objective To study the effect of Tiaogan Jiedu Formula(TJF) on learning and memory in rats with chronic epilepsy and expression of cAMP responsive element binding protein(CREB) in hippocampus.Methods Totally 60 chronic epilepsy model rats were randomly and equally divided into model group,TJF large-dose group,TJF small-dose group and Sodium Valproate group after intraperitoneal injection of Picrotoxin.The observation and treatment lasted for 60 days,and normal group of 15 rats were observed simultameously .The ability of learning and memory was tested by Morris water maze.The expression of CREB mRNA in hippocampus and level of phosphorylated CREB in hippocampus were detected by reverse transcription polymerase chain reaction and immunohistochemical method respectively.Results Memory ability,relative absorbance of CREB mRNA and positive expression of CREB immunocytes in TJF large-dose and small-dose groups increased significantly as compared with model group(P<0.01 or P<0.05).Conclusion TJF can improve learning and memory ability of rats with chronic epilepsy,which might be relevant to its regulating effect on the expression of CREB in hippocampus.更多还原