【摘要】 通过摇瓶发酵,研究温度、摇瓶转速、碳源种类和浓度、氮源种类和浓度对热带假丝酵母(Candida tropicalis)产酸的影响.结果表明:菌体生长的适宜温度为30℃,而菌体产酸的适宜温度则为32℃;摇瓶的适宜转速为210 r/min;菌体发酵的适宜外加碳源为蔗糖,适宜浓度为4g/L;菌体发酵适宜外加氮源为酵母膏和尿素,适宜浓度均为2 g/L.更多还原

【Abstract】 By the fermentation in shake flask,it was studied that the influence of temperature,the shaking speed of shake flask,the kind and concentration of carbon source and the kind and concentration of nitrogenous source on the production of dicarboxylic acid by fermentation of Candida tropicalis.The result indicated that the cell can grow well at 30 ℃ and reach the highest productivity of dicarboxylic acid at 32 ℃;the optimal shaking speed is 210 r/min;the addition of 4 g/L sucrose in the base medium can improve the fermentations;the addition of 2 g/L yeast extract and urea in the base medium is beneficial to increase the productivity.更多还原