【摘要】 目的:观察氧化苦参碱(oxymatrine,OMT)在动物实验中对阿霉素(ADR)所致心脏毒性的保护作用。方法:26只新西兰大白兔随机分为4组:ADR组(8只)、ADR+OMT组(8只)、生理盐水组(5只)、OMT组(5只)。实验兔每周耳缘静脉注射ADR(2mg/kg)1次,共8周,制备慢性心肌损伤模型。OMT(10mg/kg)于ADR应用前30min经耳缘静脉注射。用药前和停药3周后左颈总动脉及左室插管测定左室射血分数(LVEF)、左心室收缩压(LVSP)和左心室舒张末压(LVEDP);取血测定血液中乳酸脱氢酶(LDH)和肌酸磷酸激酶(CK-MB)活性。停药3周后,电镜观察心肌细胞超微结构变化。结果:ADR组中LVEF、LVSP明显降低,LVEDP明显升高,LDH和CK-MB活力显著升高。ADR+OMT组也有相似改变,程度较轻,两组之间差异有统计学意义。ADR组和ADR+OMT组均有心肌超微结构损伤,但ADR+OMT组病变程度明显轻于ADR组。OMT组动物未观察到任何不良反应和病理变化。结论:氧化苦参碱对阿霉素致心肌损伤具有保护作用。更多还原

【Abstract】 Objective:To observe the protective effect of oxymatrine(OMT)against chronic cardiotoxicity induced by adriamy- cin(ADR).Methods:Twenty six rabbits were randomly devided into four groups.The ADR group(n=8)were injected with ADR (2mg/kg)via auricular vein once a week for 8 weeks to induce chronic heart injury.The ADR+OMT group(n=8)received intrave- neous injection of OMT 10mg/kg at 30 min before administration of ADR.The normal saline group(n=5)received the same volume of 0.9% saline alone and the OMT group(n=5)received intraveneous injection of OMT alone.Before administration and 3 weeks after drug therapy,a catheter was advanced through left carotid artery and left ventricle to measure heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),and left ventricular injection fraction(LVEF).The serum was co- llected for determination of the activities of lactate dehydrogenase(LDH)and creatine kinase isoenzyme MB(CK-MB).The myocardial ultrastructure changes were observed by electron microscropy at 3 weeks after drug therapy.Results:Compared with ADR+OMT group,both LVSP and LVEF in ADR group were signi-ficantly decreased,but LVEDP and the activities of LDH and CK-MB were sig- nificantly increased.The difference between the two groups was significant.Pathological examination demonstrated the more severer da- mage of the myocardial uhrastructure in ADR group than ADR+OMT group.No adverse reaction and pathological changes were ob- served in OMT group.Conclusion:Oxymatrine protects heart against adriamycin-induced injury in rabbits.更多还原