【摘要】 目的:探讨人乳头状瘤病毒(human papilloma virus,HPV)各亚型在新疆维吾尔族宫颈癌患者及正常人群中的分布及其差异,分析维吾尔族宫颈癌的HPV谱。方法:采用导流杂交基因芯片技术,对330例新疆维吾尔族宫颈癌患者及100例正常妇女宫颈组织中的HPV21种亚型(包括13种高危亚型,5种低危亚型和3种中国人群常见亚型)进行检测。结果:(1)330例宫颈癌组织中HPV(包括单一感染及多重感染),阳性率为85.15%(281/300);100例对照组中HPV阳性率为7.0%(7/100)。HPV16(94.31%),HPV18(5.34%),HPV68(3.91%),HPV45(2.49%),HPV58(2.49%),HPV39(2.14%),HPV31(1.07%)HPV56(1.07%),HPV59(0.36%)。比较两组HPV总感染率及HPV16的阳性率,两者差异有显著性(P<0.0001)。(2)HPV21种亚型中12种被检测到,9种未检测到。HPV单一感染率及总感染率在宫颈鳞癌及其他类型的宫颈癌中的比例明显高于宫颈腺癌(P<0.05),而多重感染在各病理类型的宫颈癌中的比例无显著性(P>0.05)。HPV的多重感染在Ⅰ期,Ⅱ期及Ⅲ期中的比例虽然有逐渐上升趋势,但统计学上无显著性的差异。结论:新疆维吾尔族宫颈癌患者及正常人群中以HPV16感染为主,其次为HPV18,HPV68等,HPV68可能是新疆维吾尔族较易感染的类型,体现了维吾尔族HPV感染类型的独特性。更多还原

【Abstract】 Objective: To investigate the difference in distribution of subtypes of Human papilloma virus(HPV) between cervical cancer patients and normal controls of Xinjiang Uighur women and analyze the HPV spectrum in Xinjiang Uighur women suffered from cervical cancer.Methods: Three hundred and thirty Uighur women with cervical cancer and one hundred normal healthy women were recruited in this study.Flow-through hybridization and gene chip(HybriMax) technology was used to detect the twenty one subtypes of HPV.Results:(1) The positive rate of HPV infection including simple infection and multiple infection was 85.15%(281/300) and 7.0%(7/100) in cervical cancer group and control group,respectively.The positive rate of HPV16 infection was 94.31% indicating it was the most common infection in HPV-positive cervical cancer patients.The infection rate was 5.34% for HPV18,3.91% for HPV68,2.49% for HPV45,2.49% for HPV58,2.14% for HPV39,1.07% for HPV31,1.07 for HPV56,and 0.36 for HPV59.There was significant difference in the total infection rate and the HPV16 infection rate between cervical cancer patients and controls(P<0.0001).(2) Twelve out of twenty one HPV subtypes were detected and the rest nine subtypes were not.The positive rate of simple infection and total infection of HPV was significantly higher in squamous cervical cancer tissues and other types of cervical cancer tissues compared with that in cervical adenocarcinoma tissues(P<0.05).While the proportion of HPV multiple infection had no statistical difference between different types of cervical cancer(P>0.05).Although the positive rate of multiple infection of HPV tended to increase but the difference was not significant.Conclusion: HPV16 infection was the most common in cervical cancer patients and normal controls of Xinjiang Uighur women.The next most common types of HPV were HPV18 and HPV68.Xinjiang Uighur women had the relatively higher risk of suffering HPV68 infection indicating the specificity of HPV infection in Uighur women.更多还原