【摘要】 采用体积排阻色谱(SEC-HPLC)和激光光散射(LLS)研究了由醇洗豆粕制备的不同浓度的大豆分离蛋白热诱导聚集体(100℃,15 min)的分子量分布和粒径分布。SEC-HPLC检测结果表明,经热处理的蛋白溶液主要由3部分组成,即聚集体、中间体及未聚集部分;蛋白浓度为1%时,聚集体的百分含量为18.70%;蛋白浓度增加到5%时,聚集体的百分含量增加到54.15%;同时LLS的测定结果表明,蛋白溶液有不均一的粒径分布且体系浓度增加时平均粒径(Rh)由56.5 nm增至144.9 nm。更多还原

【Abstract】 Molecular weight distribution and particle size distribution of heat-induced soy protein isolate aggregates prepared from alcohol washed soy meal were researched by high performance size exclusion chromatography(SEC-HPLC) and laser light scattering(LLS),respectively.SEC-HPLC analysis showed that the heated protein solutions were composed of three major parts,i.e.,aggregate,intermediate and unaggregated molecules,and with the protein concentration increased from 1% to 5%,the aggregates fraction increased from 18.70% to 54.15%.At the same time,LLS analysis showed that there were inhomogeneous particle size distribution in protein solution,and with the protein concentration increased,the average hydrodynamic radius(Rh) increased from 56.5 nm to 144.9 nm.更多还原