【摘要】 目的利用高脂饲料诱发动脉粥样硬化(AS)大鼠模型,观察益气活血代表中药黄芪、三七有效成分黄芪槲皮素(Que)和三七总皂甙(tPNS)抗AS的作用,探讨二者单独使用及不同比例组方对AS大鼠模型血清基质金属蛋白酶特异性抑制物(TIMP-1)表达的影响。方法将64只雄性健康Wistar大鼠随机分为正常组和造模组,正常组10只喂养普通饲料,造模组54只喂养高脂饲料。造模成功后,造模组大鼠停喂高脂饲料改为普通饲料喂养,剩余48只造模组大鼠再随机分为6组,用不同药物干预灌胃30d后,取各组大鼠血清用酶联免疫吸附试验(ELISA)测血清TIMP-1的表达。结果Que、tPNS单用以及2∶1组方、3∶1组方皆可增加TIMP-1表达,以3∶1组方为最优组。结论Que、tPNS单用及2∶1、3∶1组方皆可增加TIMP-1表达,联合用药比单独应用抗AS效果好,并在一定范围内随着益气药物剂量增加,抗AS作用增强。这可能是益气活血法抗AS作用机制之一。更多还原

【Abstract】 Objective To explore the effect of quercetin (Que) and PNS (tPNS) on the metalloproteinase (TIMP-1) expression.Methods Sixty-four healthy male Wistar rats were randomly divided into two groups: Normal group (n=10) and model group (n=54). The rats in model group were randomly divided into six groups. TIMP-1 expression was tested by ELISA. Results Que and tPNS alone or combination of them could promote the expression of TIMP-1. Conclusion Que and tPNS could promote the expression of TIMP-1.更多还原