【摘要】 目的探讨宫颈病变程度与人类乳头状瘤病毒(HPV)16、18型负荷的关系。方法采用实时荧光定量聚合酶链反应(FQ-PCR)方法,检测宫颈不同病变活检组织及石蜡包埋标本中HPV16、18型感染状况。结果正常宫颈上皮感染HPV16、18型阳性率为3%,CINⅠ、Ⅱ、Ⅲ级和宫颈癌感染HPV16、18阳性率分别为45.7%,69.5%,72.2%和74.6%。正常宫颈与CIN,宫颈癌HPV16、18阳性率差异有极显著性(P<0.0001)。宫颈糜烂(Ⅰ°、Ⅱ°、Ⅲ°),CIN(Ⅰ、Ⅱ、Ⅲ级)及鳞癌HPV16、18阳性标本平均拷贝数分别为:3.58×103、1.80×105、1.19×107,随着病变程度加重而逐渐增加,差异有极显著性(P<0.0001)。结论宫颈病变程度与HPV16、18型负荷呈正相关。对宫颈病变组织标本行HPV16、18型检测,对判断宫颈病变发展趋势,积极处理癌前病变,阻断病程、预防宫颈癌的发生将起到重要作用。更多还原

【Abstract】 Objective:The association between human papillomavirus(HPV)16,18 type DNA copy number and grade of cervical disease was investigated.Methods: Real time fluorescence quantitative PCR(FQ-PCR),which combines PCR and fluorescence probe hybridization,was used to measure HPV 16,18 DNA copy number in 606 cases specimens of different cervical disease biopsy and embeded in paraffin.Results: Normal cervical epithelium HPV16,18 DNA was detected positive 1 case(1/32,3%),copy number 5.0×102,erosion of cervix positive 35 cases(35/241,14.5%),average copy number 3.58×103,cervical intraepithelial neoplasia(CIN) positive 56 cases(56/100,56%),average copy number 1.80×105,squamous cell carcinoma positive 168 cases(168/225 74.7%),average copy number 1.19×107.The cellular HPV16,18 DNA copy number increased significantly with increasing grade of cervical disease(P<0.0001).Conclusion: Grade of cervical disease are associoated with human papillomaviruses 16,18 type load.Detected HPV16,18 DNA plays a very important role in diagnosis and treatment of different cervical disease.更多还原