【摘要】 目的观察溃结灵对实验性大鼠溃疡性结肠炎的治疗作用。方法采用三硝基苯磺酸灌肠法制作溃疡性结肠炎大鼠模型,溃结灵采用高、中、低3个剂量(18.3,9.2,4.6g/kg)给药,治疗结束时眼眶采血,酶联免疫法检测血清IL-1β含量。处死动物取结肠,肉眼观察肠黏膜的病变并按损伤程度积分,制作病理切片,HE染色,光镜下评价黏膜损伤指数。结果溃结灵高、中剂量组组织损伤积分分别为2.41±0.79和2.83±0.71,明显少于模型组(3.75±0.75)(P<0.01);光镜下观察溃结灵高、中剂量组肠黏膜病理损害程度明显轻于模型组(P<0.05或P<0.01);溃结灵高、中剂量组血清样本中IL-1β的含量明显低于模型组血清样本(P<0.01)。结论溃结灵对溃疡性结肠炎大鼠模型有较好的治疗作用,其作用机制可能与其降低血清IL-1β含量有关。更多还原

【Abstract】 Objective To observe the therapeutic effect of Kuijieling Decoction on experimental ulcerative colitis rats.Methods Ulcerative colitis rat model was induced by enteroclysis with trinitro-benzene-sulfonic acid(TNBS).Three dosages(18.3 g/kg,9.2 g/kg,4.6 g/kg)of Kuijieling Decoction were used to treat the model rats.After treatment,the serum of rats were obtained to detect the level of IL-1β by enzyme-linked immunosorbent assay.Then the rats were killed for the observation of the colonic pathologic changes by naked eye and for the estimation of the damage scores of colon mucosa.Finally,the colon tissue was fixed to make pathological section and the damage index of colon mucosa was observed under microscope.Results The tissue damage scores of high-and moderate-dosage groups of Kuijieling Decoction were(2.41± 0.79)and(2.83± 0.71)respectively,and were significantly lower than that of model control group(3.75± 0.75;P < 0.01).Observed under microscope,the pathological damage was significantly relieved in high-and moderate-dosage Kuijieling Decoction groups as compared with that in the model control group(P < 0.05 or P < 0.01).The serum level of IL-1β in high-and moderate-dosage Kuijieling Decoction groups was significantly lower than that in the model control group(P < 0.01).Conclusion Kuijieling Decoction has protective effect against rat ulcerative colitis,and its mechanism may be related with the reduction of IL-1β .更多还原