【摘要】 目的:研究甘草抗病毒有效部位(GC3-1-4)体外抑制副流感病毒(Ⅲ型)的作用。方法:采用煎煮法和柱层析法提取分离得到甘草抗病毒有效部位(CC3-1-4)。以病毒唑为阳性对照药,采用细胞病变抑制实验观察CC3-1-4在Hela细胞中对副流感病毒(Ⅲ型)的抑制作用。结果GC3-1-4半数申毒浓度(TQ50))为144．17μg／ml;抑制副流感病毒(Ⅲ型)的半数有效浓度(EC50)为12.82μg/ml,治疗指数(TI)为11．25;GC3-1-4对副流感病毒(Ⅲ型)的抑制作用存在量效反应关系;在感染后6h以內给药,GC3-1-4对副流感病毒(Ⅲ型)均有抑制作用。结论:GC3-1-4在Hela细胞中对副流感病毒(Ⅲ型)有抑制作用。更多还原

【Abstract】 Objective: To study the activity against Parainfluenza Virus - 3 of the Active Compound (GC3 - 1 - 4) from Radix Glycyrrhizae in vitro. Methods: The active component GC-3-1-4was extracted from Glycyrrhiza by hot water extraction, ethanol precipitation and column chromatography. The inhibitory effect was observed in Hela cells using Ribavirin as a positive control. Results: The median toxic concentration (TC50), median efficacious concentration (EC50) and treatment index (Tl)of GC3-1-4 were 144.17μg/ml,12.82μg/ml, 11.25, respectively. It showed inhibitory activity on PIV when GC3 - 1 - 4 was added Oh, 2h, 4h, 6h after infections. Conclusion: The study suggested that the GC - 3 - 1 - 4 has an obvious inhibitor to Parainfluenza Virus - 3 in Hela cell culture.更多还原