【摘要】 目的探讨表皮生长因子受体(EGF-R)反义寡核苷酸转染体外培养的表皮角质形成细胞株HaCaT后,对紫外线诱导的表皮角质形成细胞转录因子c-jun活性的影响。方法用一种高灵敏度、高特异性的比色法测定不同剂量中波紫外线(UVB)辐射后以及EGF-R反义寡核苷酸转染后紫外线辐射的角质形成细胞c-jun活性的变化;RT-PCR方法测定EGF-R反义寡核苷酸转染后EGF-RmRNA的表达。结果10、20、30mJ/cm2UVB辐射角质形成细胞后均可显著增强c-jun活性(P<0.05),不同浓度的EGF-R反义寡核苷酸转染后对30mJ/cm2UVB诱导的EGF-RmRNA表达和c-jun活性均有显著抑制作用(P<0.01)。结论脂质体介导的EGF-R反义寡核苷酸转染表皮角质形成细胞可以抑制UVB辐射诱导的表皮角质形成细胞c-jun活性,表明紫外线诱导角质形成细胞c-jun激活是通过EGF-R介导的。更多还原

【Abstract】 Objective To explore the effects of antisense epidermal growth factor receptor(EGF-R)oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.Methods c-jun DNA binding activity after ultraviolet-B(UVB)irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method.After EGF-R oligodeoxynucleotides transfection,the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.Results Compared with control groups,c-jun activity increased significantly in UVB(10,20,30mJ/cm2)irradiation groups(P<0.05).EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2,4 and 8 μg/ml concentrations(P<0.01).Conclusion The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides,which is transfected to keratinocytes and mediated by lipofectamine.更多还原