【摘要】 目的研究不同剂量下染铅大鼠血铅、尿铅的时间变化效应,探讨通过腹腔注射染毒复制预防性排铅大鼠模型的适宜剂量。方法选用Wistar大鼠32只,随机分成4组:阴性对照组、低剂量组、中剂量组和高剂量组,分别给予腹腔注射乙酸铅溶液0.3 ml/100 g(剂量分别为每毫升溶液中含Pb2+0、11、546、1092μg),隔天注射1次,共7次。观察大鼠的中毒症状,每周末称体重,在染铅期间和停止染铅后定期检测大鼠血铅和尿铅含量。结果染铅1周后,3个剂量组血铅含量明显升高,中、高剂量组尿铅排出量迅速增加并达到高峰;停止染铅后,低剂量组血铅迅速降低,而中、高剂量组血铅仍处于较高的水中并持续到实验结束,尿铅排出量逐渐下降,到停铅5周时降到对照组的水平。结论过高剂量染铅使铅在体内蓄积,尿铅排出持续时间长,影响排铅制剂的排铅效果观察。建议以每毫升含546μg Pb2+为染毒适宜剂量。更多还原

【Abstract】 [Objective]To study the time change effect of blood lead and urine lead in rats that exposed to different dosages of lead and find out the proper dosage for lead egestion through abdomen injection.[Methods]32 Wistar rats were divided into four groups,namely,negative control group,low dosage group,medium dosage group and high dosage group,and were given abdomen injection of acetic acid lead of different dosages(0.3 ml/100g.bw;0,11,546 and 1092 μg/ml Pb2+ respectively) every other day for 7 times;the poisoning symptoms and the weight of the rats were observed;blood lead and urine lead of the rats were detected before and after poisoning.[Results] One week after the poisoning,the blood lead of the three dosage groups increased significantly;urine lead egestion of the medium and high dosage groups reached the peak amount;when the lead poisoning stopped,the blood lead of the low dosage group decreased rapidly,while that of the medium and high dosage groups were still quite high until the end the experiment,but the urine lead egestion decreased until to the level of the control 5 weeks after the end of the poisoning.[Conclusion] High dosage lead poisoning causes lead accumulation in body,longer lead egestion period and influence the observation of the lead egestion;it is suggested that the proper dosage for lead egestion is 546μg /ml Pb2+.更多还原