【摘要】 目的:建立以高效液相色谱法测定刺五加浸膏中紫丁香树脂苷(苷E)含量的方法。方法:色谱柱YMC-ODS柱,流动相为甲醇-水(20∶80),检测波长λs=220nm,流速1.0L/min,柱温30℃,进样量10μL。结果:苷E在0.0168~0.084μg(r=0.9993)范围内呈良好的线性关系。平均回收率为97.43%(RSD=1.21%)。结论:本方法简便、准确、重现性好。更多还原

【Abstract】 Objective: To establish a method of HPLC determination of Syringaresinol in Extractum Acanthopanacis Senticosi. Methods: YMC-ODS column was used with a mobile phase of methanol-water (20 ∶ 80);detector wavelength Syringaresinol 220nm; flow rate 1.0mL/min;column temperature at 30℃;injecting volume 10μl. Results: Syringaresinol showed the good linear relationship at the range of 0.0168μg～0.084μg(r=0.9993)respectively;The average recovery was:97.43%(RSD=1.21%) respectively. Conclusion: The method simple, accurate and reproducible.更多还原