【摘要】 目的探讨电焊作业中产生的臭氧是否引起电焊作业者体内氧化应激和氧化损伤。方法采用分光光度法检测电焊作业者(WOs)血浆维生素C(VC)、维生素E(VE)含量,红细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)活性和过氧化脂质(LPO)含量。结果与健康组比较,电焊作业者组的血浆VC和VE均值以及红细胞SOD、CAT和GPX平均值显著降低,而红细胞LPO均值显著升高。随着电焊作业者接触臭氧时间的延长,除CAT之外,VC、VE、SOD和GPX值均逐渐降低,LPO值逐渐升高。随着作业场所臭氧浓度的升高,电焊作业者VE、VE、SOD、CAT和GPX值逐渐降低,LPO值逐渐升高。实验参数的可靠性分析,可靠性系数为0.8021,P<0.0001;标化可靠性系数0.9577,P<0.001。结论电焊作业者体内存在由长期接触臭氧而引起的氧化应激,进而诱发潜在的氧化和脂质过氧化损伤。更多还原

【Abstract】 Objective To explore whether ozone induced in welding leads to oxidative stress or oxidative injury in welders.Methods The contents of vitamin C,vitamin E in plasma,the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxide(GPX) and the content of lipid peroxide(LPO) in erythrocytes were examined by spectrophotometry in welders.Results Compared with health individuals,the average values of vitamin C,vitamin E and SOD,CAT,GPX were significantly lower whereas the value of LPO was significantly higher in welders.The values of VC,VE,SOD,GPX decreased while LPO increased gradually with the extending of ozone-exposed duration in welders except CAT.The values of VC,VE,SOD,CAT,GPX decreased while LPO increased progressively as the concentration of ozone increased in the air in job locations.As the results of reliability analysis for all of the parameters,the reliability coefficient and the standardized reliability coefficient were 0.8021(P<0.0001) and 0.9577(P<0.0001), respectively.Conclusions The oxidative stress which is conduced by a long-term exposure to ozone may induce the potential oxidative and lipid peroxidative injury in welders.更多还原