【摘要】 目的:研究水蛭不同提取物对人血浆的抗凝活性及作用环节。方法:测定水蛭乙醇提取物不同萃取部位对凝血酶原时间(PT),凝血酶时间(TT),活化的部分凝血活酶时间(APTT),纤维蛋白原凝固时间(FCT)的影响。结果:水蛭的乙酸乙酯部位显著地延长人PT,APTT,TT和FCT;石油醚部位、正丁醇部位、水部位也有较弱抗凝活性。结论:水蛭乙酸乙酯提取部分抗凝作用最强,直接抑制凝血酶催化的纤维蛋白原凝固。更多还原

【Abstract】 Objective:To study the anticoagulant activity of different portions of leech ethanol extracts(LEEs).Methods:Anticoagulant activity was determined by measuring prothrombin time(PT),thrombin time(TT),activated partial throboplstin time(APTT) and fibrinogen coagulation time(FCT).Results:PT,TT,APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs.Portions of petroleum ethrer,n-butanol and water extracted from LEEs was much weaker on anticoagulant activity.Conclusion:The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions,and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.更多还原