【摘要】 目的:建立HPLC法测定麻杏甘石汤各配伍煎液中主要化学成分(麻黄碱、伪麻黄碱、甘草次酸、苦杏仁甘)的含量测定方法,并研究配伍对各化学成分含量的影响。方法:采用L8(27)正交设计实验,以HPLC法测定各化学成分的含量。结果:各配伍中主要化学成分的含量都产生一定的变化,麻黄碱、伪麻黄碱及苦杏仁苷的含量于全方去甘草组中含量显著降低;甘草次酸的含量于全方去杏仁组中也显著下降;其余各组含量变化不显著。以上4个化学成分的含量在全方组中均较高。结论:杏仁使麻黄碱、伪麻黄碱的煎出量减少,甘草有助于苦杏苷的煎出,杏仁有助于甘草酸的煎出。更多还原

【Abstract】 Objective: To set up the quantitative method of berberine in decoctions prepared from various combinations of Maxingganshi decotion by HPLC and to determine the change of general contents in different decoction. Methods: The study was designed by L8(27) orthogonal and to determine the change of general contents in different decoction by HPLC.Results: In full formula remove Glycyrrhiza uralensis group,the contents of L-ephedrine,d-pseudephedrine and the amygdaloside cut down significantly.In full formula remove armeniacae semen group,the content of glycyrrhetic acid cut down significantly.Four chemical composition in full formula group had higher contents.Conclusion: Armeniacae semen can decrease the content of L-ephedrine,D-pseudephedrine.Glycyrrhiza uralensis can help decoctum amygdaloside and armeniacae semen can help decoctum glycyrrhizic acid.更多还原