【摘要】 目的:考察反向透析法用于体内经皮吸收过程中微透析探针回收率校正的可行性。方法:在体外试验中用零净流量法测定探针的回收率和传递率,在体内试验中用反向透析法测定探针的回收率;以小猪为试验动物,观察模型药雷公藤内酯醇软膏的体内经皮吸收过程。结果:体外试验中,微透析探针的回收率为(64.29±2.34)%,回收率和传递率一致;体内经皮微透析实验中探针的回收率在实验前为(51.47±3.51)%,实验结束后为(51.96±4.97)%。雷公藤内酯醇软膏经皮吸收后,药物在皮内浓度很快达到稳态,去除药物后皮内药物浓度则迅速下降。结论:反向透析法测定的探针回收率在实验过程中稳定,可以用于测定体内经皮吸收微透析探针回收率。更多还原

【Abstract】 Objective: To assess the feasibility of retrodialysis method by calculating the recovery of microdialysis probe in percutaneous absorption in vivo.Methods:Zero-net-flux method was used to assess the recovery and delivery of probe in vitro.Retrodialysis method was used to calculate the recovery of probe in vivo.The in vivo percutaneous absorption process of triptolide was performed using pigs.Results:In the in vitro experiment,the recovery of probe was(64.29±2.34)%.The result from recovery was consistent with that from delivery.In the in vivo experiment,the recoveries of probe before and after experiment were(51.47±3.51)% and(51.96±4.97)%,respectively.The concentration of triptolide in dermis quickly reached to a steady state after administration of triptolide ointment but rapidly dropped following removal of the ointment.Conclusion:The recovery of probe calculated by retrodialysis method was stable throughout all experiments,indicating that the retrodilaysis method could be applied to assessing the recovery of probe in vivo.更多还原