【摘要】 目的:建立测定鬼臼毒素微乳凝胶剂中鬼臼毒素含量的方法。方法:采用反相高效液相色谱法,色谱柱为Lichrospher C₁₈（250 mm×4．6 mm,5μm）,以甲醇-水（63:37）为流动相,流速为0．8 mL·min^-1,检测波长为292 nm。结果:鬼臼毒素在200～800μg·mL^-1范围内呈良好的线性关系,r=0．999 9。平均回收率为99．6％,RSD为0．6％;精密度与溶液稳定性RSD分别为0．2％和0．2％。结论:方法快速,重现性、精密度及准确度均能满足鬼臼毒素含量测定要求。更多还原

【Abstract】 Objective:To establish a RP-HPLC method for the determination of podophyllotoxin in microemulsion-based gels.Methods:The RP-HPLC assay was conducted on a Lichrospher C₁₈ column （250 mm×4.6 mm,5μm） with a mobile phase composed of methanol:water （63:37） with a flow rate of 0.8 mL·min^-1 and detection at 292 nm.Results:The linearity of podophyllotoxin concentration curve was in a range of 200～800μg·mL^-1 （r=0.9999）.The average recovery was 99.6% with a RSD of 0.6%.The RSDs of the accuracy and solution stability were 0.2% and 0.2%,respectively.Conclu- sion:This rapid,accurate and reproducible RP-HPLC method was useable for quantification of podophyl- lotoxin in microemulsion-based gels.更多还原