【摘要】 目的:研究枸杞子中2-O-β-D-葡萄糖基-L-抗坏血酸（AA-2βG）对体外培养的B16黑素瘤细胞黑素合成的影响及作用机制。方法:不同浓度的AA-2βG作用于B16黑素瘤细胞,测定细胞的增殖率、酪氨酸酶活性和黑素含量,并以维生素C（Vc）作对照。结果:AA-2BG在浓度为2．96和4．44mmol·L^-1时,对B16细胞增殖抑制率可达75．99％和85．97％,对黑素合成的抑制率为32．29％和58．75％;AA-2βG对细胞生长中酪氨酸酶活性的半抑制率浓度(IC₅₀)为1．84mmol·L^-1,而Vc则为3．85mmol·L^-1,表明AA-2βG对酪氨酸酶抑制效果优于Vc。结论:AA- 2βG具有明显的抑制细胞黑素合成作用。更多还原

【Abstract】 Objective:To evaluate whether and how 2-O-β-D-glucopyranosyl-L-ascorbic acid （AA-2βG）from Fruetus lyeii inhibits melanogenesis in cultured B16 melanoma cells.Methods:A series of AA-2βG concentrations were incubated with the B16 cells.Subsequently,the inhibitory effect of AA- 2βG was assessed based on proliferation rate of the B16 cells and levels of melanin and tyrosinase in com- parison with vitamin C（Vc）control.Results:The AA-2βG at 2.96 and 4.44 mmol·L^-1inhibited the proliferation rate of the B16 cells of 75.99% and 85.97%,respectively,and the melanin synthesis of 32.29% and 58.75%,respectively,and showed an IC₅₀of tyrosinase of 1.84 mmol·L^-1in comparison of IC₅₀of vitamin C of 3.85 mmol·L^-1,the P values were statistically different in inhibitory activities of AA- 2βG from vitamin C.Conclusion:AA-2βG inhibited melanin synthesis more significantly than vitamin C.更多还原