【摘要】 目的:观察不同浓度僵蚕注射液对凝血酶诱导血管内皮细胞释放组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂-1(PAI-1)活性的影响。方法:培养新生儿脐带静脉,选取生长良好的第2~5代脐静脉内皮细胞用于实验。将大、中、小剂量僵蚕注射液(浓度分别为600、300和150 mg/L)加入5 kU/L凝血酶诱导的内皮细胞中,培养12 h后收集细胞液,用酶联免疫吸附法(ELISA)分别检测细胞培养液中PAI-1和t-PA的活性。结果:单用5 kU/L的凝血酶组具有诱导内皮细胞分泌PAI-1增加、抑制t-PA释放作用,与无凝血酶和僵蚕的空白对照组比较差异均有显著性(P均<0.01)。与凝血酶组比较,僵蚕大、中、小剂量组均能明显增加t-PA活性、降低PAI-1活性,差异均有显著性(P均<0.01);僵蚕大、中、小剂量组对凝血酶诱导内皮细胞释放t-PA的影响率分别为96.8%、95.6%和55.1%,对PAI-1的影响率分别为92.5%、85.4%和29.3%。结论:大剂量僵蚕注射液可明显抑制凝血酶诱导的内皮细胞释放作用,并能抗血栓形成。更多还原

【Abstract】 Objective: To observe the effect of bombyx batryticatus injection(僵蚕注射液) on the tissue type plasminogen activator(t-PA) and plasminogen activator inhibitor-1(PAI1) activities of human umbilical vascular endothelial cell(HUVEC) induced by thrombin.Methods: The well grown 2-5 generations of HUVECs were cultured for the study.After different concentrations of bombyx batryticatus injection(600,300 and 150 mg/L respectively) were given,the activities of PAI-1 and t-PA released by HUVEC induced by thrombin(5 kU/L) were measured with enzyme linked immunoadsorbent assay(ELISA) 12 hours after cultivation.Results: PAI-1 was increased and t-PA expression was inhibited by 5 kU/L thrombin in HUVEC compared with the blank controls without thrombin and bombys batryticatus(both P<0.01).The activities of tPA were increased and the activities of PAI-1 were reduced by different concentrations of bombyx batryticatus injection compared with thrombin group(all P<0.01),and the effect rates of high,middle and low doses of bombys batryticatus on t-PA in HUVEC induced by thrombin were 96.8%,95.6% and 55.1%,and those on PAI-1 were 92.5%,85.4% and 29.3%,respectively.Conclusion: High dose of bombyx batryticatus injection can significantly inhibit the HUVEC releasing function induced by thrombin and inhibit the coagulation process.更多还原