【摘要】 目的:探讨葛根素对失血性休克大鼠内皮细胞和抗凝血功能的影响及意义。方法:将40只成年SD大鼠随机分为假手术组、休克组、乳酸林格液复苏组(林格组)和乳酸林格液加葛根素注射液治疗组(葛根素组)4组,每组10只。假手术组仅行颈动脉插管,不放血;失血性休克模型制备方法按Lam son法快速放血使动物处于休克状态。林格组和葛根素组于休克后1h分别回输失血量3倍的乳酸林格液或30mg/kg葛根素注射液加乳酸林格液。休克3h时取血观察血浆凝血酶调节蛋白(TM)、组织因子(TF)、抗凝血酶活性(AT:A)和蛋白C活性(PC:A)的变化。结果:与假手术组比较,休克组和林格组TM和TF含量均显著增高,AT:A和PC:A均显著降低(P均<0.01);葛根素组TM含量略有升高,TF、AT:A和PC:A略有下降,均接近假手术组水平;与林格组比较,葛根素组TM和TF含量均明显下降,AT:A和PC:A均明显升高(P均<0.01)。结论:失血性休克可导致大鼠内皮细胞损伤,抗凝血酶和蛋白C呈消耗性下降;葛根素可有效调整失血性休克后大鼠凝血和抗凝血系统失衡的发生。更多还原

【Abstract】 Objective: To investigate the effect of puerarin(葛根素) on endothelial cell and anticoagulant function of Sprague Dawley(SD) rats with hemorrhagic shock.Methods: Forty SD rats were randomly divided into four groups: sham operation,shock,lactate Ringer′s solution and puerarin groups,and each group consisted of 10 SD rats.Intubation without bloodletting was performed in sham operation group.By way of Lamson bloodletting,the rats with hemorrhagic shock were obtained.One hour after shock,three times of the volume of the lost blood of lactate Ringer′s solution was infused into the lactate Ringer′s solution group,while the same above volume of a mixture of 30 mg/kg puerarin injection and lactate Ringer′s solution was infused into the puerarin group.Blood specimens were collected 3 hours after shock.Changes of plasma thrombomodulin(TM),tissue factor(TF),protein C activity(PC:A) and antithrombin activity(AT:A) of the four groups were detected.Results: The levels of plasma TM and TF in shock group and Ringer′s solution group were obviously higher than those of sham operation group(all P<0.01),and the levels of plasma AT:A and PC:A in shock group and Ringer′s solution group were clearly lower than those of sham operation group(all P<0.01).The level of plasma TM in puerarin group was slightly increased,and the levels of plasma TF,AT:A and PC:A were lowered;all above items were similar to those of sham operation group.The levels of plasma TM and TF in puerarin group were obviously lower than those of Ringer′s solution group,and the levels of plasma AT:A and PC:A in puerarin group were obviously higher than those of Ringer′s solution group(all P<0.01).Conclusion: The rats with hemorrhagic shock have trauma of endothelial cells and decrease of protein C and antithrombin due to consumption.Puerarin could effectively regulate the imbalance between the coagulant and anticoagulant functions of rats with hemorrhagic shock.更多还原