【摘要】 目的:通过检测慢性病毒性肝炎患者血清基质金属蛋白酶抑制因子-1(TIMP-1)、透明质酸(HA)、型前胶原氮端肽(PNP)、型胶原(C)、层粘连蛋白(LN)水平,分析TIMP-1、HA与肝纤维化的关系,探讨二者对肝纤维化的诊断价值。方法:酶联免疫法(ELISA)检测40例慢性病毒性肝炎患者及12例健康体检者血清TIMP-1,放射免疫法(RIA)检测其血清HA、PNP、C、LN水平,同时对患者行肝穿刺活检,以病理诊断为金标准,分析各项血清学指标与肝纤维化分期及炎症分级的关系。结果:血清TIMP-1在肝纤维化早期即明显升高,且随肝纤维化程度的加重而逐渐升高(P<0.01);血清HA在肝纤维化中后期升高;二者均与肝纤维化分期成正相关(rs分别为0.878、0.828,P<0.01)。结论:血清TIMP-1能区分肝纤维化不同时期,适于动态观察;血清TIMP-1、HA可作为肝纤维化无创性诊断的指标。更多还原

【Abstract】 Objective:To study the relationship between serum Tissue Inhibitor of Metalloproteinase-1(TIMP-1)、hyaluronic acid(HA) in patients with chronic viral hepatitis and liver fibrosis.Methods:Concentrations of serum TIMP-1、HA、N-terminal peptide of type Ⅲ procollagen(PⅢNP)、type Ⅳ collagen(CⅣ)、laminin(LN) in 40 patients with chronic viral hepatitis and 12 healthy subjects were measured by Enzyme-linked immunoassay or radioimmunoassay.Liver biopsies were performed in all of the patients at the same time. According to the results of liver pathological diagnosis,the correlations of serum markers and the degrees of liver fibrosis were assessed.Results:Concentrations of serum TIMP-1 and HA rose in the early stage and were statistically different in various stages of liver fibrosis.The correlation coefficients of serum TIMP-1 and HA levels with the stages of fibrosis were 878、0.828.Conclusions:Serum TIMP-1 can differentiate various stages of liver fibrosis. Serum TIMP-1、HA can be regarded as useful markers for liver fibrosis.更多还原