【摘要】 目的:探讨异基因造血干细胞移植(allo-HSCT)后巨细胞病毒感染的早期诊断及人类巨细胞病毒血症(Cy-tom egalov irus an tigenem ia)与巨细胞病毒病(CMV d isease)发生的关系。方法:CMV pp65单克隆抗体荧光免疫组织化学法检测CMV早期抗原,并进行动态观察。结果:47例HSCT中,17例(36.17%)发生巨细胞病毒血症,7例(14.89%)发生CMV病。基础疾病为血液系统恶性疾病的患者,进行异基因造血干细胞移植后,巨细胞病毒抗原血症和巨细胞病毒病的发生率分别为54.55%和31.82%,而血液系统非恶性疾病的患者分别为20.00%和0.00%,两者之间差异有显著性(P<0.05)。基础疾病、急性GVHD是巨细胞病毒抗原血症和巨细胞病毒病发生的危险因素。结论:CMV pp65病毒抗原检测在造血干细胞移植后CMV病的早期诊断、早期预防、早期治疗及延长移植后长期生存率具有重要意义。更多还原

【Abstract】 Objective:To investigate the relationship between cytomegalovirus antigenemia,CMV disease and related risk facrtors in patient undergoing allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:CMVpp65 antigen in leukocytes was detected by indirected immunoflurenscence assay using CMV Brite Kit.Results:Among 47 patients,CMV antigenemia developed in 17 patients (36.17%) and CMV disease in 7(14.89%),The frequencies for antigenemia and CMV disease were 54.55%,31.82% and 20.00%,0.00%,respectively in hematological malignancies and non-malignancy.(P<0.05).Underlying disease and acute GVHD were found to be a significant risk factors for CMV antigenemia and disease.Conclusion:The detection of CMVpp65 antigen plays an important role in not only early diagnosis,prevention,treatment for CMV antigenemia and disease,but also extending survallence in patients undergoing allogeneic hematopoietic stem cell transplantation(allo-HSCT).更多还原