【摘要】 利用ODS-C18反相高效液相色谱体系研究了东北红豆杉(Taxus cuspidata)叶、茎、果肉及种子中紫杉醇含量的时空动态变化。结果表明,东北红豆杉的4个部位均有紫杉醇存在,但存在的时间和部位具有较大差异。紫杉醇的含量最大值为0.043mg.g-1(FW)出现在10月份种子中,最小值0.001mg.g-1(FW)出现在11月份果肉中。叶与茎中紫杉醇的含量变化较为一致,叶和茎中紫杉醇含量在7月份达到最高,分别为0.032mg.g-1(FW)和0.029mg.g-1(FW),在8月份降至最低,分别为0.011mg.g-1(FW)和0.010mg.g-1(FW)。在新叶萌发期,老茎叶比新茎叶中紫杉醇的含量高约2.5倍。更多还原

【Abstract】 The present study examined time and space variation of paclitaxel content in needles, stems, pulp and seeds of Taxus cuspidata by use of ODS-C18 RP-HPLC; this article describes the accumulation and metabolism of paclitaxel in T. cuspidata. Although paclitaxel was in all of four organs of T. cuspidata, its level was highest during development and differed among organs. Maximal fresh-weight paclitaxel content was 0.043 mg.g-1 in seeds in October, and minimal content was 0.001 mg.g-1 in pulp in November. Needles and stems showed a similar pattern of paclitaxel content: the maximal content in needles and stems was 0.032 mg.g-1 and 0.029 mg.g-1, respectively, in July, and the content decreased to 0.011 mg.g-1 and 0.010 mg.g-1, respectively, in August. During growth, the content in the old needles and stems was approximately 2.5-fold that in young needles and stems.更多还原