【摘要】 选择性微电极技术是一种不仅能直接测定活的生物细胞或细胞器内的离子或分子活度,而且能对活的生物相邻的位置、功能和代谢速率可能不同的特定微区细胞表面的离子或分子流(flux)分别测定的电生理方法。具有操作简便、实时、非损伤性(测定离子或分子流)、灵敏度高(可达10-12molescm-2s-1)等优点。因为它是用微型化(尖端直径为0.5~5μm)的离子或分子选择性电极直接对准样品测定,不同于其他化学测定需取样品,所以能连续测定和自动监测,具有广阔的应用前景。该文阐述了选择性微电极测定原理,总结了选择性微电极技术在植物生理学研究中的应用进展,并展望了其发展前景。更多还原

【Abstract】 Selective microelectrode technique, known as an electrophysiological approach, can be used to measure directly specific information on ion or molecule distribution and movement both inside and outside of living organelle, biological cells, tissue and organs. It has several advantages over other methods in measuring ionic or molecu- lar information, e.g. easy to handle, fast response, high sensitivity (10-12 moles cm-2 s-1) and non-in- vasive to the samples in addition to continuous measurement and automatic monitoring. Micro- scopic-scale selective electrode (with a tip diam- eter of 0.5–5 μm) can be used to measure net fluxes of ions or molecules outside of growing biologicalcells, tissues and organs, to measure activities of ions or molecules inside of growing organelle and biological cells. Thus, it has many applications in various research fields. The technical principle of design and use of selective microelectrode and its progress and development prospect in plant physi- ological research are summarized.更多还原