【摘要】 目的建立脑胶质瘤的组织培养药敏检测法,用于筛选化疗药物及指导临床个体化化疗。方法应用组织培养药敏检测法对69例人脑胶质瘤标本进行化疗药物敏感性测定,所测药物为脑肿瘤常用化疗药物顺铂(DDP)、长春新碱(VCR)、替尼泊甙(VM26)、尼莫司汀(ACNU)、替莫唑胺(TMZ)及DDP+VM26组合。比较6组化疗药物体外抗肿瘤作用的差异,并分析药物敏感性与病人临床资料之间的相关性。结果DDP+VM26组合用药的敏感性与VM26单独用药比较,差异无统计学意义,但明显优于其他4种单药。病人性别、年龄、肿瘤初发或复发均对药物敏感性无显著性影响;除组合用药DDP+VM26外,其他药物的敏感性与病理分级无显著相关性。结论胶质瘤标本不同个体对同一药物及同一个体对不同药物的敏感性差异很大,脑胶质瘤化疗应优先选择以VM26为主的联合用药。更多还原

【Abstract】 Objective To select chemotherapeutic agents for guiding tailor-made chemotherapy by chemosensitivity test of glioma cells in tissue culture.Methods Sixty-nine specimens of brain glioma were used to evaluate the chemosensitivity of glioma cells by tissue culture in vitro.The chemotherapeutic agents tested for brain cancer were cisplatin(DDP),vincristine(VCR),teniposide(VM26),nimustine(ACNU),temozolomide(TMZ) and DDP+VM26 group.The antineoplastic effects of these agents were compared,and the correlations between the chemosensitivity and patient characteristics were analyzed.Results There was no significant difference in the chemosensitivity between of DDP+VM26 and VM26 alone,but both of them were significantly higher than the other four groups.There was no significant influence of patients’ sex and age and oncogenesis or relapse of the tumors on chemosensitivity.Except DDP+VM26 group,the other drugs showed no significant correlation of chemosensitivity with the pathological grade of the tumor.Conclusion In vitro chemosensitivity of glioma varies with not only the individuals but also the chemotherapeutic agents,and combined agents containing VM26 are more effective for glioma treatment.更多还原